Confocal Laser Scanning Microscopy Evaluation of the Effect of Lentulo Spiral and Ultrasonic Activation on Intra-tubular Sealer Penetration

Document Type : Original Article

Abstract

 Objective: To evaluate the effect of lentulospiral and ultrasonic activation on the intra-tubular AH plus sealer penetration
using confocal laser scanning microscopy.
Materials and Methods: Twenty extracted single-rooted teeth were
collected; teeth were prepared up to ProTaper finishing file F4 (size 40). Teeth were randomly divided into two main
groups (n=10 teeth) according to activation techniques. The AH plus sealer with rhodamine B was activated by
lentulospiral in group 1 and Irrisafe ultrasonic tip in group 2. The root canals were obturated with a single gutta-percha
cone (size F4). The roots were sectioned at 3, 6, and 9 mm levels from the apex and examined with confocal laser
scanning microscopy. Statistical analyses were performed at a 5% significance level.
Results: At the cervical third, the
lentulospiral group had significantly higher means of maximum depth (828.2±274.1 μm) and percentage (49.72±8.96
%) and area (2774.9±908.6 μm
2) of sealer penetration (P<0.05). While the Irrisafe group had significantly higher means
of maximum depth (486.9±269.9 μm) and percentage (24.34±13.21 %) sealer penetration at the apical third (P<0.05).
There was a positive correlation between the maximum depth (μm) and the area (μm2) of sealer penetration (P < 0.01
and confidence of 99 %).
Conclusions: Regarding sealer activation, lentulospiral performed better in the cervical region
while activated Irrisafe ultrasonic tip performed better in the apical region.
 

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