Invitro Evaluation of Bioceramic Sealer Penetration in Radicular Dentin: A Confocal Microscopy Study

Document Type : Original Article

Abstract

 Objective: To assess the area of sealer penetration into dentinal tubules in different sections of
the root canal using a confocal laser scanning microscope after irrigation with NaOCl and followed by ultrasonic
activation. Materials and Methods: Mandibular premolars were selected according to the inclusion criteria which are:
single straight root canal or slightly curved, fully developed apex with patent foramina, no obstruction in the canal
system or indication of internal and/or external resorption or signs of crack or fracture, then the teeth were prepared,
irrigated using NaOCl, activated, and then filled with Meta Biomed CeraSeal Bioceramic sealer using a single cone
obturation technique. Rhodamine B was added to the sealer. Specimens were sectioned and divided into coronal
sections, middle sections, and apical sections. The percentage of the sealer's penetration area was determined for each
root level using a 10x confocal laser microscope. Results: The results were displayed as mean ±SD. A statistically
significant difference was found between the studied sections (P < 0.001). Coronal sections presented the largest sealer
penetration (35.19%), while apical sections showed the lowest sealer penetration. Conclusions: Penetration is affected
by root dentine level as bioceramic sealer shows inferior penetration at smaller distances from the apex.

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