Comparison of Self-Assembling Peptide with Different Remineralizing Agents on Demineralized Primary Molars: In-Vitro Study

Objective: Evaluating the remineralization potential of Self assembling peptide (P11-4), Nano-hydroxyapatite (Nano-HA) and Casein Phosphopeptide-Amorphous Calcium Phosphate-Fluoride (CPP-ACPF) using Scanning Electron Microscopy (SEM) and Energy dispersive X-ray Analysis (EDX). Materials and Methods: Forty enamel specimens of primary second molar were prepared and immersed in demineralizing solution for 96 hours to induce artificial carious lesions. Specimens were divided randomly according to remineralizing agents into four equal groups. The groups were named group A: control group (artificial saliva), group B: P11-4, group C: Nano-HA paste and group D: CPP-ACPF. Remineralization was carried out for a period of 21 days. Calcium and Phosphorous content was evaluated using EDX-SEM for all specimens. The data were collected and statistically analyzed using One Way ANOVA test. Results: The Calcium/Phosphorous ratio % after remineralization was significantly higher with Self assembling peptide and Nano-HA and the increase in CPP-ACPF was non-significant compared to control group. On SEM examination, there was a restoration of enamel surface with appearance of mineralized deposits. It was highly observed in group B and group C then group D and the lowest was in group A. Conclusion: Self-assembling peptide and Nano-hydroxyapatite were found to be more effective compared to CPP-ACPF in remineralization.