Biomimetic remineralization of artificial dentinal carious lesions using two different bioactive capping materials.

doi:10.21608/mjd.2019.199474

Biomimetic remineralization of artificial dentinal carious lesions using two different bioactive capping materials.

Document Type : Original Article

10.21608/mjd.2019.199474

Abstract

Purpose: This study was conducted to evaluate the remineralization potential of two bioactive pulp capping materials (Biodentine ^TM and Lime-lite ^TM) in the presence of biomimetic analogs (Poly-acrylic acid and Sodium trimetaphosphate).
Materials and Methods: Two standardized occlusal cavities (mesial and distal) were prepared within dentin surfaces with the dimensions of 2 mm in diameter & 0.5 mm in depth after removal of occlusal enamel. Artificial demineralized dentin was induced though pH cycling (8 hours in demineralizing solution & 16 hours in remineralizing solution). Whole occlusal dentin was protected from pH cycling except the cavities and a small rim (1 to 2 mm) surrounding them. Demineralized cavities were divided into four groups; the first two groups received capping materials. The other two were first treated with biomimetic analogs prior to material application. Teeth were sectioned buccolingually into two halves. Teeth halves that received treatment with analogs were stored in simulated body fluid containing poly-acrylic acid. Un-treated cavities were stored in simulated body fluid only. The specimens were examined after 1, 6 and 12 weeks storage using Energy dispersive x-ray spectroscopy (EDX) and Scanning electron microscope (SEM). Two-way ANOVA was used to analyze data statistically.
Results: Specimens received Biodentine with biomimetic analogs showed the highest statistically significant calcium and phosphorous Wt% after 12 weeks of storage. Scanning electron microscope images showed filling of intra-tubular dentin at the interface region in all tested groups.
Conclusion: Biodentine showed the best ability to enrich the artificial carious dentin with significantly higher Wt % of ions for remineralization especially in the presence of biomimetic analogs. Both tested materials have the ability to obliterate open dentinal tubules after total period of storage.