Impact of Two Different Application Times of A Natural Collagen Crosslinker on The Bonding Quality to Caries Affected Dentin

Abdelshafia, Mostafa A.; Fathyb, Salma M.; Osmanc, Manal F; Abbas Ali Elkhoolyd, Tarek; Mohamed Reichaf, Fikry

doi:10.21608/mjd.2020.198744

Impact of Two Different Application Times of A Natural Collagen Crosslinker on The Bonding Quality to Caries Affected Dentin

Document Type : Original Article

Authors

¹ Assistant Lecturer of Dental Biomaterials, Faculty of Dentistry, Mansoura University.

² Associate professor of Dental Biomaterials, Faculty of Dentistry, Zagazig University.

³ Professor of Dental Biomaterials, Faculty of Dentistry, Mansoura University.

⁴ Associate professor of biophysics and biomaterials, Biochemistry Department, Faculty of medicine, Delta University for Science and Technology.

⁵ Professor of Experimental Solid State Physics, Faculty of Science, Mansoura University.

10.21608/mjd.2020.198744

Abstract

Objectives: The current study was conducted to evaluate a natural collagen crosslinker as a dentin pretreatment step for improving the bond strength to caries affected dentin (CAD) at two different application timing.
Materials and Methods: Fifteen molars had their coronal dentin exposed followed by artificial caries induction. Following, dentin was etched for 10 seconds and treated with either 6.5% grape seed extract (GSE) or phosphate buffered solution (PBS) for either 10 minutes or 1 hour. Samples were bonded and restored with Single Bond Universal Adhesive (SB) and Z250 XT nanohybrid composite resin. The samples were sectioned into resin dentin sticks and tested for microtensile bond strength immediately and after thermocycling. Data were statistically analysed using three way-ANOVA followed by t-test (α = 0.05).
Results: Higher immediate 𝜇TBS were observed for 1 hour GSE when compared to 10 minutes GSE and control groups, but non-significant (𝑃> 0.05). After thermocycling, results showed non-significant reduction in 𝜇TBS for either times of GSE treatment (𝑃> 0.05). In contrast, significant reduction in 𝜇TBS for control group was observed (𝑃 ≤ 0.05).
Significance: GSE may be effective in enhancing the bond strength to CAD with increasing the concentration and application time. Both treatment times were effective in maintaining the bond strength after thermocycling.